Chemometec YC-100 Bedienungsanleitung

NucleoCounter YC-100 User's Guide Revision 1.2 Technology that counts

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Introduction and intended use xIntroduction and intended use The NucleoCounter YC-100 is intended to be used for the determination of yeast cell

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1Warnings and precautions Wherever the symbol appears on the NucleoCounter instrument, it indicates that the manual must be consulted for preca

2Table Table Table Table 2222 Description of the NucleoCounter Printer Output connector Pin noPin noPin noPin no NameNameNameName Maxi

3 The user shall be made aware of that, if the NucleoCounter and the external power supply is used in a manner not specified by the manufacturer,

4Never pipette by mouth. Avoid cross contamination of the samples when preparing of the samples. This can compromise the quality of the results.

5 Table of contents DECLARATION OF CONFORMITY ... VI WEEE DIRECTIVE

65.4.1 Result mode ... 21 5.4.2 Saving parameters ...

1 Inspection and Unpacking of Equipment 71 Inspection and Unpacking of Equipment Upon receiving the order from ChemoMetec A/S, the box or boxes shou

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1 Inspection and Unpacking of Equipment 8 This page was intentionally left blank

2 Introducing the NucleoCounter 92 Introducing the NucleoCounter YC-100 The NucleoCounter method provides a fast alternative to the manual technique

2 Introducing the NucleoCounter 102.1.1 Fluorescence microscope The compact fluorescence microscope integrated in the NucleoCounter comprises LED´s (

2 Introducing the NucleoCounter 11A fluorescent image of the content of the measurement chamber is thus . Example of such an image is shown in Figure

2 Introducing the NucleoCounter 12 Figure Figure Figure Figure 5555 The NucleoCassette. The fluorescent dye, propidium iodide, is immobilized in the

2 Introducing the NucleoCounter 13by multiplying the imaged area with the depth of the cassette. The imaged area is only dependent on the optics of t

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3 Control Buttons, Keypad and Display 153 Control Buttons, Keypad and Display 3.1 Interactive Controls 3.1.1 Control Buttons There are four Control

3 Control Buttons, Keypad and Display 163.2 Interface Display 3.2.1 Status indicator A small Status Indicator light above the “On/Off” button (see F

4 Installation and Start-up 174 Installation and Start-up 4.1 Power on, power off The NucleoCounter is powered by a 12VDC external power supply. To

ii NucleoCounter YC-100Yeast Cell CounterP / N 9 9 1 - 0 3 0 0 ( E n g l i s h )R e v i s i o n 1 . 2D e c e m b e r , 2 0 0 5

4 Installation and Start-up 18After a successful start-up the display changes (Figure 9) and the status indicator on the keyboard panel turns green.

5 Operation of the NucleoCounter 195 Operation of the NucleoCounter YC-100 5.1 Ready mode After the NucleoCounter has started successfully the disp

5 Operation of the NucleoCounter 20After analysis the lid is lifted and the cassette is removed from the insertion slit and disposed of according to

5 Operation of the NucleoCounter 215.3.3 External computer If an external computer with the NucleoView software installed on it is connected, the res

5 Operation of the NucleoCounter 22• Press <↵>. 5.4.2 Saving parameters The representation of the results is changed with effect from the next

5 Operation of the NucleoCounter 23 Contrast? 6 Contrast? 6 Contrast? 6 Contrast? 6 1: Up1: Up1: Up1: Up

5 Operation of the NucleoCounter 24 Setup? haploidSetup? haploidSetup? haploidSetup? haploid 1:Up1:Up1:Up1:Up

5 Operation of the NucleoCounter 25elevated signal levels. If the number of pixels is above a predefined limit then the cassette insertion slit can b

5 Operation of the NucleoCounter 26After a few seconds the instrument has performed the Zero Count Check. If no contamination of the insertion slit c

6 Total and viability count 276 Total and viability count The NucleoCounter detects fluorescent signals from propidium iodide (PI) when it is bound

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6 Total and viability count 28 6.3 Determination of Viability When both the concentration of non-viable cells and the total concentration of cells a

7 Maintenance of NucleoCounter 297 Maintenance of NucleoCounter YC-100 A regular cleaning of the NucleoCounter is recommended in order to protect it

7 Maintenance of NucleoCounter 30 Figure Figure Figure Figure 26262626... An image showing a contamination of the optical system, visible as a

7 Maintenance of NucleoCounter 317.1.4 Spill of liquid If liquid has been spilled on the instrument it can contaminate elements of the optical system

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8 Troubleshooting - Error messages 33 8 Troubleshooting - Error messages Under certain conditions the NucleoCounter will display error messages duri

8 Troubleshooting - Error messages 34 8.3 Actuator error messages The actuator moves the piston down into the barrel of the cassette. Two different

8 Troubleshooting - Error messages 35 Sensor errorSensor errorSensor errorSensor error Refer to manualRefer to manualRefer to manualRefer to

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9 Technical specifications 37 9 Technical specifications 9.1 Technical specifications for NucleoCounter YC-100 Specificity Cells stained with the

ivCaution!Caution!Caution!Caution! This equipment must be operated as described in this User’ s Guide. Please read the entire guide before attemp

9 Technical specifications 38 Other environmental operating conditions for the NucleoCounter Indoor use only. Altitude Up to 2000 m. INSTALLATI

39 TabTabTabTable le le le 4444... Equipment and Accessories for NucleoCounter system Item Part no. Description NucleoCounter YC-100 900-0300

40 Item Part no. Description using ink ribbon, for the NucleoCounter Thermo Printer PCNEOS-S2BN 939-0006 Optional thermo printer, using thermo p

41 UL listed, type SVT, rated min. 60C, 18 AWG, 3 conductors. Provided with molded-on grounding-type (NEMA 5-15P) attachment plug, rated min. 125 Va

42 10.5 External Printer (optional) An external impact or thermo printer may optionally be connected to the NucleoCounter for the printing of t

43 Metal Enclosure Figure Figure Figure Figure 36363636 Printer Output connector seen from the cable entry side.

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45 11 Appendix 1: WEEE directive information in more EU languages United Kingdom Correct Disposal of This Product (Waste Electrical & Electron

46 Norway Korrekt avhending av dette produkt (Avfall elektrisk og elektronisk utstyr) - Kun Europa Denne merkingen som vises på produktet eller d

47 Finland Tämän tuotteen turvallinen hävittäminen (elektroniikka ja sähkölaitteet) - Vain Eurooppa Oheinen merkintä tuotteessa tai tuotteen ohei

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48 The information contained herein is to the best of our knowledge accurate and complete. However cell species and ce

viDeclaration of Conformity Name of product: NucleoCounter Type: YC-100 Other identifying data: Part no. 900-0300 The product com

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viiiWEEE directive information – Europe only Correct Disposal of This Product (Waste Electrical & Electronic Equipment) - Europe only This mar